Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 3. (A) Effect of temperature and endocytosis inhibitors on the internalization of HA-Se@PTX in A549 cells. p < .05, p < .01 vs. control group. (B) In vitro release of PTX from HA-Se@PTX nanoparticles. p < .01 vs. pH5.4 group.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques: Control, In Vitro

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 2. Cellular uptake of HA-Se@PTX in A549 cells (A) and HUVEC (B) was observed by fluorescence microscope, respectively. Scale bar is 20 lm. (C) Quantitative uptake of SeNPs, HA-SeNPs and HA-Se@PTX in A549 cells was analyzed by ICP-MS. (D) The uptake of HA-Se@PTX in A549 cells untreated/pretreated with HA were tested by ICP-MS.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques: Fluorescence, Microscopy

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 5. (A) The wound width of A549 cells was photographed after 12 h of co-incubation with PTX, Se@PTX and HA-Se@PTX, respectively. Scale bar is 400 mm. (B) The quantitative analysis of cell motility in control, PTX, Se@PTX and HA-Se@PTX groups. (C) The effect of PTX, Se@PTX, and HA-Se@PTX on the migration of A549 cells. Scale bar is 200 mm. (D) The quantitative analysis of cell migration inhibition rate in PTX, Se@PTX, and HA-Se@PTX groups. p < .05 vs. PTX group.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques: Incubation, Control, Migration, Inhibition

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 4. (A) The cytotoxicity of PTX, Se@PTX and HA-Se@PTX at various PTX equivalent concentrations against A549 cells. (B) The cytotoxicity of HA-SeNPs against A549 cells. p < .05, p < .01 vs. PTX group.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques:

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 7. The protein expression of MMP-2, MMP-9, cleavage-caspase-3, and cleavage-PARP in A549 cells exposed to different concentrations of HA-Se@PTX.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques: Expressing

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Hyaluronic acid-modified selenium nanoparticles for enhancing the therapeutic efficacy of paclitaxel in lung cancer therapy.

doi: 10.1080/21691401.2019.1626863

Figure Lengend Snippet: Figure 6. (A) Effect of PTX, Se@PTX, and HA-Se@PTX on the cell cycle distribution and apoptosis in A549 cells, respectively. (B) Apoptosis percentage analysis of A549 cells after exposed to PTX, Se@PTX, and HA-Se@PTX, respectively.

Article Snippet: Non-small cell lung carcinoma A549 cells and human umbilical vein endothelial cells (HUVEC) were obtained from American Tissue Culture Collection (ATCC) and were cultivated in DMEM with 10% FBS in an incubator (80% humidity, Thermo Scientific, South Logan, USA) with 5% CO2 at 37 C.

Techniques: